Interested in RNA Biology research? Take a look at what MCDB faculty are doing in this area:
Mechanisms underlying heart and skeletal muscle diseases, with a primary focus on the role of RNA-binding proteins in regulating the expression of pathologic genes during stress challenges.
Interested in how cells become sequentially determined to more precisely defined fates during vertebrate embryonic development and how this process depends upon cell position and upon interactions among neighboring cells. To address these questions, we use genetics, molecular biology, time-lapse imaging, and embryology to investigate mesodermal patterning, segmentation and muscle development in the zebrafish embryo, a well-established model for human development and disease.
Molecular mechanisms of bacterial gene expression and its control by nucleic acid signals, regulatory proteins, and antibiotics.
Geminivirus replication, gene expression, and pathogenesis. The role of small RNA-directed epigenetic silencing in defense against DNA viruses.
Our studies use genetically engineered model systems to gain insight into the molecular processes that govern biological aging and the development of cancer.
Cancer biology; regulation of pre-mRNA splicing in normal cellular function and disease.
My lab is focusing on understanding the role of non coding RNAs in leukemogenesis and acute graft versus host disease.
Role of DNA methylation and microRNAs in liver disease.
Biochemistry and applications of ribonuclease P, a catalytic RNP complex.
Dr. Guo's lab is really interdisciplinary with diverse technologies and variable projects involving the areas of cell biology, molecular medicine, virology, biophysics, biotechnology, biochemistry, chemistry, computation, biomedical engineering, single molecular optics, single molecular conductance, single pore sensing, RNA Nanotechnology, nucleic acid chemistry, cancer therapy, drug delivery, viral DNA packaging, and ATPase motors. The lab has been focused on the study of viral DNA packaging motor that is composed of a protein channel driven by six ATPase and geared by six RNA molecules.
NF-kappa β regulation of cell growth and differentiation.
Regulation of gene expression; Bacillus subtilis; grampositive bacteria; transcription antitermination; transcription activation and repression; RNAstructure and function; tRNA.
Heritable epigenetic variation represents a poorly understood, yet significant, component of evolutionary biology. We use genetic, cytogenetic, genomic, and molecular approaches in corn to understand both the molecular mechanism and biological function of paramutation - a process responsible for meiotically heritable epigenetic changes in gene regulation. Our studies highlight novel aspects of eukaryotic chromosome organization and function.
Intracellular trafficking of RNA and proteins; Nucleus organization; RNA processing.
Our research is directed towards understanding the mechanisms that determine how cells ensure the accurate translation of the genetic code, and how changes in the underlying processes impact cellular health and contribute to microbial pathogenesis and disease. Many of these processes are essential and unique to particular systems, making them ideal potential drug targets.
The Jackman laboratory utilizes the principles and techniques of mechanistic enzymology and enzyme kinetics to elucidate the molecular mechanisms of tRNA processing enzymes.
Understand HIV replication, cancer development and DNA repair processes at the molecular level, using mass spectrometry as both a proteomic and structural biology tool to elucidate the composition and architecture of key biological macromolecules.
Cell biology of osteoclasts with particular emphasis on differentiation and the cytoskeleton; mechanisms by which kidney tubule cells regulate mRNA levels during and following cellular stresses.
Assembly of viral and host cell RNAs into HIV-1 and other retroviruses; Quality control by aminoacyl-tRNA synthetases and related trans-editing enzymes; Non-canonical functions of aminoacyl-tRNA synthetases and role in human disease.
Elucidating the role of microRNAs and epigenetic aberrations in multiple myeloma (MM). Lab is also focused on studying the role of MM microvesicles in cell-cell communication and on preclinical evaluation in experimental therapeutics for clinical trials.
Molecular and cellular mechanism of intracellular parasitism/Ehrlichia spp.
The laboratory utilizes mouse models of human cancer to investigate the role of parathyroid hormone-related protein in bone metastasis and cancer-associated hypercalcemia. Metastases are monitored using in vivo bioluminescence of luciferase-transfected tumor cells. Molecular studies are focused on the regulation of PTHrP mRNA stability by transforming growth factors.
Regulation of mRNAstability; pre-mRNA processing and translation initiation; posttranscriptional control by estrogen.
Slotkin, R. Keith
My laboratory aims to discover how potentially mutagenic “jumping genes” or transposable elements are epigenetically repressed from generation to generation, as well as how this system has been adopted over evolutionary time to regulate non-transposable element genes.
Causes and consequences of endogenous transposition and alternative RNA splicing in mouse and man.
Post-transcriptional gene regulation in Drosophila; germ cell biology.
Molecular biology of the human major histocompatibility complex class III products and eukocyte antigen CD1.