The Neginskaya Laboratory investigates the molecular mechanisms of mitochondrial dysfunction during cellular stress and injury. Our research focuses on how changes in mitochondrial inner membrane permeability contribute to cell and tissue damage during ischemia–reperfusion injury in the heart and brain. We combine mitochondrial patch-clamp electrophysiology, advanced imaging, biochemical approaches, and structural methods to study the mechanisms that regulate mitochondrial inner membrane permeability. 

The traditional view of pathological mitochondrial permeability transition (mPT) describes it as the opening of a single large, nonselective pore in the inner mitochondrial membrane. Our research challenges this paradigm by identifying multiple permeability pathways within the inner mitochondrial membrane and investigating their distinct molecular mechanisms and physiological roles. Current projects examine the functions of mitochondrial proteins that regulate these pathways during cellular stress and injury. 

Our long-term goal is to identify new molecular targets and therapeutic strategies to preserve mitochondrial function and reduce tissue injury.